USDA-Approved Plate Antigens for Mycoplasma and Salmonella

Mycoplasma gallisepticum  Antigen (USDA PRODUCT CODE: 5071.02)

SPAFAS MG Plate Antigen for Plate Agglutination Test  (Catalog No. 551301)

This antigen is a suspension of killed, stained A5969 strain of Mycoplasma gallisepticum (MG) for serum plate agglutination (SPA) testing of chicken and turkey flocks. It has been standardized against a reference antigen and a panel of known positive and negative sera.

Mycoplasma synoviae Antigen (USDA PRODUCT CODE: 5073.02)  

SPAFAS MS Plate Antigen for Plate Agglutination Test  (Catalog No. 551401)

This antigen is a suspension of killed, stained WVU1853 strain of Mycoplasma synoviae (MS) for serum plate agglutination (SPA) testing of chicken flocks. It has been standardized against a reference antigen and a panel of known positive and negative sera.

Salmonella pullorum  Antigen (USDA PRODUCT CODE: 5207.00)

LAH Pullorum Stained Antigen for Plate Agglutination Test  (Catalog No. 551601)

This antigen is a suspension of five (5) killed, stained strains (4, 11, 77, 79, and 296) of Salmonella pullorum  for serum plate agglutination (SPA) testing of chicken and turkey flocks. It has been standardized against a reference antigen and a panel of known positive and negative sera.

SPA Testing Method

The serum plate agglutination (SPA) test is used to detect specific antibodies that will bind to an antigen and cause visible “clumping” or agglutination. In this test, the antigen is prepared from cultures of MG or MS and a dye is added to improve visibility of the reactions. A prescribed amount of antigen is placed on a solid support such as a plastic or glass plate or mirror, keeping each drop of antigen separate. An equal amount of test serum is placed next to the antigen and these are then mixed together. After a short (2 minute) incubation, the mixture is examined for evidence of agglutination, which appears as discrete clumps of the stained particles with a clearer background. If no antibodies are detected, the mixture will remain opaque. The SPA is a screening test and positive reactions should be confirmed by other methods, such as Hemagglutination Inhibition or isolation of the organism from appropriate samples.

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